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"A la Carte" Mode
Alignment
T-Coffee
->
Curation
Gblocks
->
Phylogeny
PhyML
->
Tree Rendering
TreeDyn
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Input Data

Upload your set of sequences in FASTA, EMBL or NEXUS format from a file:

Or paste it here (load example of sequences)

Maximum number of sequences is 50 for proteins and 50 for nucleic acids.
Maximum length of sequences is 2000 for protein and 2000 for nucleic acids.



Alignment: T-Coffee

Advanced Settings...


Pairwise methods: Pairwise alignment methods will be used to make a pairwise alignment of all the possible pairs of sequences.
Lalign_pair: 10 best local alignments
Slow_pair: accurate global alignment
Fast_pair: fast heuristic global alignment
Clustalw_pair: ClustalW pairwise alignment
Mlalign_id_pair Mclustalw_pair Mfast_pair Mslow_pair

Additional alignment formats:
GCG / MSF
PIR Alignment
Pir Sequences (unaligned)
Score in ASCII format (text)


Print residues number:

Output order:



Curation: Gblocks

Settings


For a less stringent selection:
Allow smaller final blocks
Allow gap positions within the final blocks
Allow less strict flanking positions
For a more stringent selection:
Do not allow many contiguous nonconserved positions



Phylogeny: PhyML

Settings


Statistical tests for branch support:
Approximate Likelihood-Ratio Test (aLRT):   
Bootstrapping procedure:    Number of bootstraps:   

Substitution model:   


Advanced Settings...


Number of substitution rate categories:
Gamma distribution parameter: estimated     fixed:    
Proportion of invariable sites: estimated     fixed:    
Transition / transversion ratio (nucleic acids only): estimated     fixed:    
Remove gaps from alignment    



Tree Rendering: TreeDyn

Settings


Tree can be customized using the dynamic tree editing interface.



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